Develop hands-on expertise in cloning, transformation, and recombinant protein expression workflows, with exposure to real R&D laboratory environments.
Course Curriculum
Module 1: Core Laboratory Training
Micropipetting precision and buffer preparation
Sterilization techniques (autoclaving vs filtration)
Media preparation (LB, TB, SOB)
Aseptic techniques and culture handling
Competent cell preparation (CaCl₂ method)
Module 2: Cloning & Expression Workflows
Plasmid isolation (Miniprep)
Restriction digestion and ligation
Transformation (E. coli systems)
Colony PCR screening
Protein expression (IPTG induction)
Optimization (temperature, time, concentration)
Growth monitoring (OD600)
Module 3: Downstream Processing & Purification
Cell harvesting and sonication (lysis optimization)
Ni-NTA affinity chromatography
Dialysis and buffer exchange
Protein concentration techniques
Module 4: Analytics & Characterization
Protein quantification (BCA / NanoDrop)
SDS-PAGE (purity analysis)
ELISA basics (specificity testing)
Data interpretation
Module 5: Process Optimization
Troubleshooting cloning and expression issues
Solubility strategies (inclusion bodies vs soluble proteins)
Multi-step purification workflows
Resin regeneration and reuse
Module 6: Scale-Up & Stability
Scale-up (100 mL to 2L cultures)
Stability studies
Lyophilization basics
Cryoprotectant strategies
Module 7: Lab Management & Compliance
Inventory and consumable planning
Equipment calibration & maintenance
Preventive maintenance (FPLC, incubators, LAF systems)